Friday, May 20, 2016

Utilization of Cell-Transfer Technique for Molecular Testing on Hematoxylin-Eosin–Stained Sections

Utilization of Cell-Transfer Technique for Molecular Testing on Hematoxylin-Eosin–Stained Sections
A Viable Option for Small Biopsies That Lack Tumor Tissues in Paraffin Block

Howard H. Wu MDStephen M. Jovonovich MDMelissa Randolph CT(ASCP)Kristin M. Post MLS(ASCP), MPHJoyashree D. Sen MDKendra Curless MLS(ASCP)Liang Cheng MD
Reprints: Howard H. Wu, MD, Department of Pathology and Laboratory Medicine, Indiana University School of Medicine, 350 W 11th St, IUHPL-Room 4086, Indianapolis, IN 46202 (email: ).
Context.—In some instances the standard method of doing molecular testing from formalin-fixed, paraffin-embedded block is not possible because of limited tissue. Tumor cell–enriched cell-transfer technique has been proven useful for performing immunocytochemistry and molecular testing on cytologic smears.
Objective.—To establish the cell-transfer technique as a viable option for isolating tumor cells from hematoxylin-eosin (H&E)–stained slides.
Design.—Molecular testing was performed by using the cell-transfer technique on 97 archived H&E-stained slides from a variety of different tumors. Results were compared to the conventional method of molecular testing.
Results.—Polymerase chain reaction–based molecular testing via the cell-transfer technique was successfully performed on 82 of 97 samples (85%). This included 39 of 47 cases for EGFR, 10 of 11 cases for BRAF, and 33 of 39 cases for KRASmutations. Eighty-one of 82 cell-transfer technique samples (99%) showed agreement with previous standard method results, including 4 mutations and 35 wild-type alleles for EGFR, 4 mutations and 6 wild-type alleles for BRAF, and 11 mutations and 21 wild-type alleles for KRAS. There was only 1 discrepancy: a cell-transfer technique with a false-negativeKRAS result (wild type versus G12C).
Conclusions.—Molecular testing performed on H&E-stained sections via cell-transfer technique is useful when cell blocks and small surgical biopsy samples run out of tissue and the only available material for testing is on H&E-stained slides.

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