From Marshall Austin and colleagues: Screening Test Results Associated With Cancer Diagnoses in 287 Women With Cervical Squamous Cell Carcinoma

http://www.archivesofpathology.org/doi/pdf/10.5858/arpa.2011-0609-OA

Screening Test Results Associated With Cancer Diagnoses in 287 Women With Cervical Squamous Cell Carcinoma

Zaibo Li, MD, PhD; R. Marshall Austin, MD, PhD; Ming Guo, MD; Chengquan Zhao, MD

Context.—The limitations of newer cervical screening tests are still being studied.
Objective.—To investigate those limitations, we conducted a retrospective review of 287 cases of cervical
squamous cell carcinoma (SCC).
Design.—A search through 5 years of records identified 287 women with cervical SCC diagnoses. Clinical history, histopathology, and liquid-based cytology (ThinPrep) and Hybrid Capture 2 (HC2) high-risk human papillomavirus (HPV) results were documented. Polymerase chain reaction HPV tests were performed on SCC tissues with prior negative HC2 results.
Results.—Of 287 women, 156 (54.4%) had abnormal cytology results, and 75 (26.1%) had abnormal clinical findings triggering tissue diagnoses of SCC. Among 156 patients with abnormal Papanicolaou (Pap) test results, more-seriously abnormal Pap test results were reported in 142 women (91.1%). Among 31 women with Pap and HC2 HPV cotesting within 1 year of SCC diagnoses, 28 (90%) were Pap + /HPV + ,   (15.5%) were Pap + /HPV2 , and 1 (3%) was Pap2 /HPV2 . Two of 3 women with negative HC2 results before SCC diagnosis had abnormal Pap results; 1 had negative Pap reports with questionable lesional cells
mimicking atrophy. In all 3 cases of SCC with negative HC2 results, HPV-18 was detected; in 2 cases (66%), HPV- 16 was also detected.
Conclusions.—Although abnormal cytology preceded most SCC diagnoses, about one-third of patients were
referred for diagnostic testing because of clinical suspicion alone. Among 31 SCC cases with liquid-based cytology and high-risk HPV cotest results less than 1 year before SCC diagnoses, 2 patients (6.5%) had Pap
+ /HPV2 results, and 1 patient (3.2%) had Pap2 /HPV2 results. Polymerase chain reaction detected high-risk HPV DNA in tumor tissues of 3 SCC cases with recent HC22 results.


(Arch Pathol Lab Med. 2012;136:1–8; doi: 10.5858/
arpa.2011-0609-OA)